Anti-ARHGEF12 Antibody (PACO55278)

Antibody Name:	ARHGEF12 Antibody (PACO55278)
Antibody SKU:	PACO55278
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Rho guanine nucleotide exchange factor 12 protein (1302-1501AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO55278 at dilution of 1:100.
	Immunofluorescent analysis of HepG2 cells using PACO55278 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human testis tissue using PACO55278 at dilution of 1:100.

Background:	May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
Synonyms:	Rho guanine nucleotide exchange factor 12 (Leukemia-associated RhoGEF), ARHGEF12, KIAA0382 LARG

UniProt Protein Function:	ARHGEF12: a widely-expressed guanine nucleotide exchange factor (GEF) for RhoA GTPase. May act as GTPase-activating protein (GAP) for G-alpha 12 and 13. The fusion protein MLL-ARHGEF12 appears to be a disease driver in acute myeloid leukemia. Two isoforms of the human protein are produced by alternative splicing.Protein type: GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis; OncoproteinChromosomal Location of Human Ortholog: 11q23.3Cellular Component: cytoplasm; cytosolMolecular Function: G-protein-coupled receptor binding; guanyl-nucleotide exchange factor activity; protein binding; Rho guanyl-nucleotide exchange factor activityBiological Process: G-protein coupled receptor protein signaling pathway; positive regulation of apoptosis; regulation of small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:	Q9NZN5
NCBI GenInfo Identifier:	34395525
NCBI Gene ID:	23365
NCBI Accession:	Q9NZN5.1
UniProt Secondary Accession:	Q9NZN5,O15086, Q6P526
UniProt Related Accession:	Q9NZN5
Molecular Weight:	173kDa
NCBI Full Name:	Rho guanine nucleotide exchange factor 12
NCBI Synonym Full Names:	Rho guanine nucleotide exchange factor 12
NCBI Official Symbol:	ARHGEF12
NCBI Official Synonym Symbols:	LARG; PRO2792
NCBI Protein Information:	rho guanine nucleotide exchange factor 12
UniProt Protein Name:	Rho guanine nucleotide exchange factor 12
UniProt Synonym Protein Names:	Leukemia-associated RhoGEF
Protein Family:	Rho guanine nucleotide exchange factor
UniProt Gene Name:	ARHGEF12
UniProt Entry Name:

Antibodies

ARHGEF12 Antibody (PACO02767)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)