The ARHGEF12 Polyclonal Antibody (PAC019922) is a valuable tool for researchers studying ARHGEF12, a protein involved in cell signaling and cytoskeletal organization. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to ARHGEF12, this antibody allows for the detection and analysis of the protein in a variety of cell types, making it ideal for studies in cell biology and signal transduction research.ARHGEF12, also known as Rho guanine nucleotide exchange factor 12, plays a crucial role in regulating the activity of Rho GTPases, which are key regulators of cell motility and shape.
Dysregulation of ARHGEF12 has been implicated in various diseases, including cancer and neurological disorders. Understanding the function of ARHGEF12 is essential for uncovering the molecular mechanisms underlying these conditions and developing targeted therapies.Overall, the ARHGEF12 Polyclonal Antibody provides researchers with a reliable tool for investigating the function and regulation of ARHGEF12, offering valuable insights into its role in cellular processes and disease pathways.
Antibody Name:
ARHGEF12 Antibody (PACO19922)
Antibody SKU:
PACO19922
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human ARHGEF12
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO19922(ARHGEF12 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO19922(ARHGEF12 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms:
Rho guanine nucleotide exchange factor (GEF) 12
UniProt Protein Function:
ARHGEF12: a widely-expressed guanine nucleotide exchange factor (GEF) for RhoA GTPase. May act as GTPase-activating protein (GAP) for G-alpha 12 and 13. The fusion protein MLL-ARHGEF12 appears to be a disease driver in acute myeloid leukemia. Two isoforms of the human protein are produced by alternative splicing.Protein type: GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis; OncoproteinChromosomal Location of Human Ortholog: 11q23.3Cellular Component: cytoplasm; cytosolMolecular Function: G-protein-coupled receptor binding; guanyl-nucleotide exchange factor activity; protein binding; Rho guanyl-nucleotide exchange factor activityBiological Process: G-protein coupled receptor protein signaling pathway; positive regulation of apoptosis; regulation of small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
