The ARHGDIB Polyclonal Antibody (CAB3740) is a valuable research tool for studying ARHGDIB, a protein involved in cell signaling and regulation. This antibody, produced through rabbit immunization, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ARHGDIB protein, this antibody enables researchers to detect and analyze ARHGDIB expression in various cell types.ARHGDIB, also known as RhoGDI beta, plays a key role in regulating the activity of Rho GTPases, which are important for intracellular signaling and cell movement.
Dysregulation of ARHGDIB has been implicated in cancer, cardiovascular diseases, and neurological disorders, making it an attractive target for therapeutic interventions. By studying ARHGDIB expression and function, researchers can gain insights into its involvement in these disease processes and potentially identify new treatment strategies.
Product Name:
ARHGDIB Rabbit Polyclonal Antibody
SKU:
CAB3740
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARHGDIB (NP_001166.3).
Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).
Purification Method:
Affinity purification
Gene ID:
397
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ARHGDIB antibody (CAB3740) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
