The ARHGAP25 Polyclonal Antibody (CAB13664) is a valuable tool for researchers studying the ARHGAP25 protein, which plays a key role in regulating cell migration and cytoskeletal dynamics. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to ARHGAP25, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential component for studies in cell biology and cancer research.ARHGAP25, a Rho GTPase activating protein, is known for its involvement in controlling the activity of Rho GTPases, which are crucial regulators of cell morphology and motility.
Dysregulation of ARHGAP25 has been linked to various diseases, including cancer, where abnormal cell migration leads to tumor progression and metastasis. By investigating the role of ARHGAP25 in these processes, researchers can gain insights into potential therapeutic targets for combating invasive cancers and other diseases associated with aberrant cell movement.
Product Name:
ARHGAP25 Rabbit Polyclonal Antibody
SKU:
CAB13664
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1).
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).
Purification Method:
Affinity purification
Gene ID:
9938
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ARHGAP25 antibody (CAB13664) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
