Anti-ARHGAP18 Antibody (PACO56074)

Antibody Name:	ARHGAP18 Antibody (PACO56074)
Antibody SKU:	PACO56074
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human, Rat, Mouse
Immunogen:	Recombinant Human Rho GTPase-activating protein 18 protein (389-577AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Rat lung tissue, Rat kidney tissue, Mouse heart tissue. All lanes: ARHGAP18 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 75, 71 kDa. Observed band size: 75 kDa.
	Immunofluorescence staining of HepG2 cells with PACO56074 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO56074 diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1. Regulates cell shape, spreading, and migration.

Synonyms:

Rho GTPase-activating protein 18 (MacGAP) (Rho-type GTPase-activating protein 18), ARHGAP18

UniProt Protein Function:	ARHGAP18: a GTPase activator for the Rho-type GTPases, converting them to inactive GDP-bound states. Two alternatively-spliced isoforms have been described.Protein type: GAPs, Rac/Rho; GAPsChromosomal Location of Human Ortholog: 6q22.33Cellular Component: cytoplasm; cytosolMolecular Function: GTPase activator activityBiological Process: regulation of actin cytoskeleton organization and biogenesis; regulation of actin filament polymerization; regulation of cell shape; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:	ARHGAP18 belongs to a family of Rho (see MIM 165390) GTPase-activating proteins that modulate cell signaling (Potkin et al., 2009 [PubMed 19065146]).[supplied by OMIM, Apr 2010]
UniProt Code:	Q8N392
NCBI GenInfo Identifier:	296452981
NCBI Gene ID:	93663
NCBI Accession:	Q8N392.3
UniProt Secondary Accession:	Q8N392,Q58EZ3, Q6P679, Q6PJD7, Q96S64, E1P575
UniProt Related Accession:	Q8N392
Molecular Weight:	70,164 Da
NCBI Full Name:	Rho GTPase-activating protein 18
NCBI Synonym Full Names:	Rho GTPase activating protein 18
NCBI Official Symbol:	ARHGAP18
NCBI Official Synonym Symbols:	SENEX; MacGAP; bA307O14.2
NCBI Protein Information:	rho GTPase-activating protein 18
UniProt Protein Name:	Rho GTPase-activating protein 18
UniProt Synonym Protein Names:	MacGAP
Protein Family:
UniProt Gene Name:	ARHGAP18
UniProt Entry Name:	RHG18_HUMAN

Antibodies

ARHGAP18 Antibody (PACO06261)

ARHGAP18 Antibody (PACO07820)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)