The ARHGAP15 Polyclonal Antibody (PACO59101) is a valuable tool for researchers studying ARHGAP15, a protein involved in cell signaling pathways and cellular migration. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blot applications. By targeting the ARHGAP15 protein, this antibody allows for the detection and analysis of ARHGAP15 expression in a variety of cell types, making it ideal for investigations in cell biology and cancer research.ARHGAP15, a Rho GTPase-activating protein, plays a critical role in regulating the activities of Rho family GTPase proteins, which are essential for cell movement and organization.
Dysregulation of ARHGAP15 activity has been implicated in various disease processes, including cancer metastasis and cardiovascular disorders. By understanding the functions of ARHGAP15, researchers can gain insights into potential therapeutic targets for these conditions, as well as explore its role in other cellular processes.
IHC image of PACO59101 diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO59101 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59101 diluted at 1:600 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Synonyms:
Rho GTPase-activating protein 15 (ArhGAP15) (Rho-type GTPase-activating protein 15), ARHGAP15
UniProt Protein Function:
ARHGAP15: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.Protein type: GAPs, Rac/Rho; GAPsChromosomal Location of Human Ortholog: 2q22.2-q22.3Cellular Component: membrane; cytosolBiological Process: regulation of cell shape; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:
RHO GTPases (see ARHA; MIM 165390) regulate diverse biologic processes, and their activity is regulated by RHO GTPase-activating proteins (GAPs), such as ARHGAP15 (Seoh et al., 2003 [PubMed 12650940]).[supplied by OMIM, Mar 2008]
