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ARHGAP15 Antibody (PACO59101)

SKU:
PACO59101
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • ARHGAP15 Antibody PACO59101
  • ARHGAP15 Antibody PACO59101
  • ARHGAP15 Antibody PACO59101
€339

Description

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ARHGAP15 Antibody (PACO59101)

The ARHGAP15 Polyclonal Antibody (PACO59101) is a valuable tool for researchers studying ARHGAP15, a protein involved in cell signaling pathways and cellular migration. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blot applications. By targeting the ARHGAP15 protein, this antibody allows for the detection and analysis of ARHGAP15 expression in a variety of cell types, making it ideal for investigations in cell biology and cancer research.ARHGAP15, a Rho GTPase-activating protein, plays a critical role in regulating the activities of Rho family GTPase proteins, which are essential for cell movement and organization.

Dysregulation of ARHGAP15 activity has been implicated in various disease processes, including cancer metastasis and cardiovascular disorders. By understanding the functions of ARHGAP15, researchers can gain insights into potential therapeutic targets for these conditions, as well as explore its role in other cellular processes.

Antibody Name:ARHGAP15 Antibody (PACO59101)
Antibody SKU:PACO59101
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500
Species Reactivity:Human
Immunogen:Recombinant Human Rho GTPase-activating protein 15 protein (1-154AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageIHC image of PACO59101 diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageImmunofluorescence staining of A549 cells with PACO59101 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product ImageIHC image of PACO59101 diluted at 1:600 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Synonyms:Rho GTPase-activating protein 15 (ArhGAP15) (Rho-type GTPase-activating protein 15), ARHGAP15
UniProt Protein Function:ARHGAP15: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.Protein type: GAPs, Rac/Rho; GAPsChromosomal Location of Human Ortholog: 2q22.2-q22.3Cellular Component: membrane; cytosolBiological Process: regulation of cell shape; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:RHO GTPases (see ARHA; MIM 165390) regulate diverse biologic processes, and their activity is regulated by RHO GTPase-activating proteins (GAPs), such as ARHGAP15 (Seoh et al., 2003 [PubMed 12650940]).[supplied by OMIM, Mar 2008]
UniProt Code:Q53QZ3
NCBI GenInfo Identifier:28274758
NCBI Gene ID:55843
NCBI Accession:AAO34684.1
UniProt Secondary Accession:Q53QZ3,Q53R36, Q53RD7, Q53RT6, Q53SX9, Q584N9, Q6PJE6 Q86WP1, Q8IXX1, Q9NRL8
UniProt Related Accession:Q53QZ3
Molecular Weight:
NCBI Full Name:ARHGAP15
NCBI Synonym Full Names:Rho GTPase activating protein 15
NCBI Official Symbol:ARHGAP15  
NCBI Official Synonym Symbols:BM046  
NCBI Protein Information:rho GTPase-activating protein 15; rho-type GTPase-activating protein 15
UniProt Protein Name:Rho GTPase-activating protein 15
UniProt Synonym Protein Names:ArhGAP15; Rho-type GTPase-activating protein 15
Protein Family:Rho GTPase-activating protein
UniProt Gene Name:ARHGAP15  
UniProt Entry Name:RHG15_HUMAN
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ARHGAP15 Antibody (PACO07819)
Secondary Antibody
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