The Argonaute 2 Rabbit Monoclonal Antibody (CAB19709) is an essential tool for researchers studying the Argonaute 2 protein, a key player in the RNA interference pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot and immunohistochemistry applications.Argonaute 2 is a crucial component of the RNA-induced silencing complex (RISC), playing a central role in gene regulation and silencing through microRNA-mediated mechanisms. Its involvement in post-transcriptional gene silencing makes it a promising target for investigating various cellular processes, including development, differentiation, and disease pathways.
By enabling the detection and analysis of Argonaute 2 protein expression in different cell types, this monoclonal antibody offers valuable insights into the intricate mechanisms of RNA interference. Its versatility and reliability make it a valuable tool for researchers in the fields of molecular biology, genetics, and developmental biology, advancing our understanding of gene regulation and paving the way for innovative therapeutic strategies targeting Argonaute 2-related pathways.
Product Name:
Argonaute-2 Rabbit Monoclonal Antibody
SKU:
CAB19709
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Argonaute-2 (Q9UKV8).
PPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980; Ago 2; Argonaute-2
Positive Sample:
293T,U-87MG,MCF7,HepG2,Mouse kidney,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, P-body.
Calculated MW:
97kDa
Observed MW:
97kDa
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
27161
Clone Number:
ARC0189
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Argonaute-2 Rabbit mAb (CAB19709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
