The ARFGEF1 Polyclonal Antibody (CAB16483) is a valuable tool for research involving ARFGEF1, a protein involved in regulating cell membrane trafficking and organization. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications such as Western blot and immunofluorescence.ARFGEF1, also known as brefeldin A-inhibited guanine nucleotide-exchange protein 1, plays a crucial role in intracellular trafficking processes by activating small GTPases involved in vesicle trafficking and membrane remodeling.
Its involvement in cell polarization, migration, and vesicle transport makes it a key player in cellular processes implicated in cancer metastasis and neurodegenerative disorders.By targeting and detecting ARFGEF1 in different cell types, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. The ARFGEF1 Polyclonal Antibody is a reliable tool for studying the function and regulation of ARFGEF1, providing important information for the development of novel treatment strategies.
Product Name:
ARFGEF1 Rabbit Polyclonal Antibody
SKU:
CAB16483
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human ARFGEF1 (NP_006412.2).
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition.
Purification Method:
Affinity purification
Gene ID:
10565
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ARFGEF1 Rabbit pAb (CAB16483) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
