The ARAP1 Polyclonal Antibody (CAB10466) is a valuable tool for researchers studying ARAP1, a protein involved in various cellular functions such as cell migration, adhesion, and signaling. This antibody, produced in rabbits, is highly specific to human ARAP1 and has been validated for use in Western blot applications. By binding to ARAP1, this antibody enables the detection and analysis of ARAP1 protein in different cell types, making it ideal for studies in cell biology and cancer research.ARAP1, also known as ARAP1, is a multidomain protein that functions as a guanine nucleotide exchange factor (GEF) for small GTPases involved in cell migration and cytoskeletal rearrangement. It plays a critical role in regulating processes like cell adhesion, cell spreading, and cell motility, making it a key player in cellular signaling pathways.
Understanding the role of ARAP1 can provide valuable insights into various cellular processes and may have implications for the development of targeted therapies in cancer and other diseases.Overall, the ARAP1 Polyclonal Antibody is a powerful tool for researchers looking to study the function and regulation of ARAP1 in different cell types and contexts. Its high specificity and sensitivity make it a reliable choice for experiments in cell biology and cancer research, leading to a better understanding of ARAP1's role in cellular processes and its potential as a therapeutic target.
Product Name:
ARAP1 Rabbit Polyclonal Antibody
SKU:
CAB10466
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human ARAP1 (NP_001128662.1).
The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
116985
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ARAP1 antibody (CAB10466) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
