The ARAF Polyclonal Antibody (CAB0180) is a high-quality antibody specifically designed for research involving ARAF, a member of the RAF family of serine/threonine protein kinases. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.ARAF plays a crucial role in the MAPK signaling pathway, which is involved in various cellular processes such as cell proliferation, differentiation, and survival. Dysregulation of this pathway has been implicated in the development and progression of cancer, making ARAF a target of interest in cancer research.
By targeting the ARAF protein, this antibody allows for the detection and analysis of ARAF expression in different cell types, making it a valuable tool for studies in oncology and cell signaling pathways. Understanding the function and regulation of ARAF can provide valuable insights into the molecular mechanisms underlying cancer development, potentially leading to the identification of novel therapeutic targets for cancer treatment.
Product Name:
ARAF Rabbit Polyclonal Antibody
SKU:
CAB0180
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 371-606 of human ARAF (NP_001645.1).
Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of cellular protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma.
Purification Method:
Affinity purification
Gene ID:
369
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ARAF antibody (CAB0180) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 5s.
