The APRT Polyclonal Antibody (CAB5456) is a specialized tool for researchers studying APRT, a key enzyme involved in the purine salvage pathway. This antibody, raised in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the APRT protein, this antibody allows for precise detection and analysis in a variety of cell types, making it a valuable asset for studies in biochemistry and molecular biology.APRT, or adenine phosphoribosyltransferase, plays a crucial role in the recycling of purine bases in the body, contributing to nucleotide synthesis and maintaining cellular energy balance.
Dysfunction of APRT is associated with rare genetic disorders like APRT deficiency, which can lead to kidney stones and other health complications. Research on APRT is essential for understanding these conditions and developing potential therapies to address them.By utilizing the APRT Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of APRT in normal and disease states, paving the way for advancements in the fields of genetics, metabolism, and renal health.
Product Name:
APRT Rabbit Polyclonal Antibody
SKU:
CAB5456
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1).
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of 293T, using CALCA Rabbit pAb (CAB5456) at 1:2500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
