The Aprataxin Polyclonal Antibody (CAB5364) is a crucial tool for researchers studying aprataxin, a DNA repair protein essential for maintaining genomic integrity. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By targeting the aprataxin protein, this antibody enables precise detection and analysis in a wide range of cell types, making it an invaluable asset for studies in DNA repair mechanisms and related diseases.
Aprataxin is known for its role in repairing DNA damage caused by oxidative stress, chemical agents, and radiation. Dysfunction of aprataxin has been linked to neurodegenerative disorders such as ataxia with oculomotor apraxia type 1 (AOA1) and spinocerebellar ataxia with axonal neuropathy type 1 (SCAN1). By studying the function of aprataxin using this antibody, researchers can gain insights into the underlying mechanisms of these diseases and potentially develop targeted therapies.
Product Name:
APTX Rabbit Polyclonal Antibody
SKU:
CAB5364
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; APTX
Positive Sample:
22Rv1,A-549,K-562,BT-474,U-87MG,HT-29,Raji
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleolus, nucleoplasm.
Calculated MW:
41kDa
Observed MW:
40kDa
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
54840
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using APTX Rabbit pAb (CAB5364) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
