The APPL2 Polyclonal Antibody (CAB14590) is a valuable tool for researchers studying the APPL2 protein, a key player in cell signaling and intracellular trafficking processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for various research applications, including Western blotting.APPL2, also known as adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2, is involved in the regulation of cellular processes such as cell proliferation, migration, and adhesion.
By targeting the APPL2 protein, researchers can gain insight into its function and role in diseases like cancer, diabetes, and neurodegenerative disorders. The APPL2 Polyclonal Antibody facilitates the detection and analysis of APPL2 expression in different cell types, enabling in-depth investigations into its molecular mechanisms and potential therapeutic implications.
Product Name:
APPL2 Rabbit Polyclonal Antibody
SKU:
CAB14590
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human APPL2 (NP_060641.2).
Early endosome membrane, Nucleus, Peripheral membrane protein.
Calculated MW:
74kDa
Observed MW:
74kDa
The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome.
Purification Method:
Affinity purification
Gene ID:
55198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using APPL2 Rabbit pAb (CAB14590) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
