The APPL1 Antibody (CAB4606) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By targeting the APPL1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in cell signaling, metabolism, and cancer research.APPL1, also known as adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1, plays a crucial role in regulating insulin signaling, vesicle trafficking, and cell survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
APPL1 Antibody
SKU:
CAB4606
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APPL, MODY14, DIP13alpha, APPL1
Positive Sample:
SKOV3, SH-SY5Y, 293T, HL-60, Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Early Endosome Membrane, Nucleus, Peripheral Membrane Protein.
Calculated MW:
80kDa
Observed MW:
95kDa
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
Purification Method
Affinity purification
Gene ID
26060
RRID
AB_2765780
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using APPL1 Rabbit pAb (CAB4606) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
