The APPL Rabbit Monoclonal Antibody (CAB8932) is a high-quality research tool designed for the study of APPL, a key protein involved in various cellular processes. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in a variety of research applications.APPL, also known as Amyloid beta precursor protein-binding family A member 1, is a multifunctional protein that plays a crucial role in cell signaling, endocytosis, and neuronal development. The APPL Rabbit Monoclonal Antibody binds specifically to the APPL protein, allowing for the detection and analysis of APPL expression in various cell types and tissues.
Research involving APPL is important for understanding its role in diseases such as Alzheimer's, cancer, and neurodegenerative disorders. By studying the function of APPL, researchers can gain insights into the underlying mechanisms of these diseases and potentially develop targeted therapies.Overall, the APPL Rabbit Monoclonal Antibody (CAB8932) is a valuable tool for researchers studying APPL and its implications in various pathological conditions. Its high specificity and reactivity make it an essential component in immunology and disease research.
Product Name:
APPL1 Rabbit Monoclonal Antibody
SKU:
CAB8932
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human APPL11 (Q9UKG1).
Sequence:
KKSS ILQA ESKK DHEE WICT INNI SKQI YLSE NPEE TAAR VNQS ALEA VTPS PSFQ QRHE SLRP AAGQ SRPP TART SSSG SLGS ESTN LAAL SLDS LVAP D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
APPL; MODY14; DIP13alpha; APPL1
Positive Sample:
PC-12,BxPC-3,Mouse brain,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Early endosome membrane, Nucleus, Peripheral membrane protein.
Calculated MW:
80kDa
Observed MW:
90kDa
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
Purification Method:
Affinity purification
Gene ID:
26060
Clone Number:
ARC1356
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using APPL11 Rabbit mAb (CAB8932) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
