The APP Polyclonal Antibody (CAB16265) is an essential tool for researchers studying amyloid precursor protein (APP) and its implications in neurodegenerative diseases such as Alzheimer's. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for Western blot applications.APP is a transmembrane protein involved in the production of amyloid beta, a peptide that forms plaques in the brains of Alzheimer's patients. By targeting and binding to the APP protein, this antibody enables precise detection and analysis in various cell types, providing valuable insights into the mechanisms underlying neurodegeneration.
The dysregulation of APP processing is a key feature of Alzheimer's disease pathology, making it a prime target for therapeutic intervention. By utilizing the APP Polyclonal Antibody, researchers can further elucidate the role of APP in disease progression and potentially identify novel therapeutic strategies for combating Alzheimer's and related neurodegenerative conditions.
Product Name:
APP Rabbit Polyclonal Antibody
SKU:
CAB16265
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aβ42 (NP_000475.1).
Membrane, Single-pass type I membrane protein, clathrin-coated pit.
Calculated MW:
87kDa
Observed MW:
100kDa/140kDa
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
351
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (CAB16265) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
