Anti-APOBEC3F Antibody (CAB2507)
- SKU:
- CAB2507
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
Anti-APOBEC3F Antibody (CAB2507)
The APOBEC3F Polyclonal Antibody (CAB2507) is a valuable tool for researchers studying the APOBEC3F protein, an important member of the APOBEC family involved in innate immunity and viral restriction. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the APOBEC3F protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal choice for investigations in virology and immunology.APOBEC3F plays a crucial role in the body's defense against viral infections by catalyzing the deamination of cytidine residues in viral DNA/RNA, leading to hypermutation and eventual viral genome degradation.
Understanding the function and regulation of APOBEC3F is essential for unraveling the complex interplay between host immune responses and viral evasion strategies. Research into APOBEC3F could lead to the development of novel antiviral therapies and strategies for combating viral infections.Overall, the APOBEC3F Polyclonal Antibody (CAB2507) represents a valuable tool for researchers aiming to elucidate the role of APOBEC3F in innate immunity and viral restriction, furthering our understanding of host-pathogen interactions and potentially uncovering new therapeutic targets for combating viral infections.
| Antibody Name: | Anti-APOBEC3F Antibody |
| Antibody SKU: | CAB2507 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human APOBEC3F (NP_660341.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, BxPC-3, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human APOBEC3F (NP_660341.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKPH FRNT VERM YRDT FSYN FYNR PILS RRNT VWLC YEVK TKGP SRPR LDAK IFRG QVYS QPEH H |
| Gene ID: | 200316 |
| Uniprot: | Q8IUX4 |
| Cellular Location: | Cytoplasm, P-body |
| Calculated MW: | 9kDa/11kDa/45kDa |
| Observed MW: | 45kDa |
| Synonyms: | APOBEC3F, A3F, ARP8, BK150C2.4.MRNA, KA6 |
| Background: | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | APOBEC3F: Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. After being packaged into HIV-1 virions, blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA processing; Hydrolase; EC 3.5.4.- Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; ribonucleoprotein complex Molecular Function:protein binding; zinc ion binding; RNA binding; cytidine deaminase activity Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus |
| NCBI Summary: | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8IUX4 |
| NCBI GenInfo Identifier: | 161784334 |
| NCBI Gene ID: | 200316 |
| NCBI Accession: | Q8IUX4.3 |
| UniProt Secondary Accession: | Q8IUX4,Q45F03, Q6ICH3, Q7Z2N2, Q7Z2N5, B0QYD4, |
| UniProt Related Accession: | Q8IUX4 |
| Molecular Weight: | 373 |
| NCBI Full Name: | DNA dC-> |
| NCBI Synonym Full Names: | apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F |
| NCBI Official Symbol: | APOBEC3FÂ Â |
| NCBI Official Synonym Symbols: | A3F; KA6; ARP8; BK150C2.4.MRNAÂ Â |
| NCBI Protein Information: | DNA dC->dU-editing enzyme APOBEC-3F; induced upon T-cell activation; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B editing enzyme catalytic polypeptide-like 3F; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like |
| UniProt Protein Name: | DNA dC->dU-editing enzyme APOBEC-3F |
| UniProt Synonym Protein Names: | Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F |
| Protein Family: | DNA dC->dU-editing enzyme |
| UniProt Gene Name: | APOBEC3FÂ Â |
| UniProt Entry Name: | ABC3F_HUMAN |
 | Western blot analysis of extracts of various cell lines, using APOBEC3F antibody (CAB2507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using APOBEC3F antibody (CAB2507) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using APOBEC3F antibody (CAB2507) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using APOBEC3F antibody (CAB2507) at dilution of 1:100 (40x lens). |
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