The APOBEC3A Polyclonal Antibody (CAB12399) is specially designed for research involving APOBEC3A, a member of the APOBEC family of proteins known for their role in restricting retroviral replication. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the APOBEC3A protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal tool for studies in virology, cancer research, and immunology.
APOBEC3A is a key player in the innate immune response against retroviruses and retrotransposons, highlighting its importance in host defense mechanisms. Research on APOBEC3A is crucial for understanding viral evolution, host-pathogen interactions, and the development of antiviral therapies. By investigating the function and regulation of APOBEC3A, scientists can gain valuable insights into viral infections and potentially discover new strategies for combatting viral diseases.
Product Name:
APOBEC3A Rabbit Polyclonal Antibody
SKU:
CAB12399
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human APOBEC3A (NP_663745.1).
This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
200315
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with APOBEC3A using APOBEC3A Rabbit pAb (CAB12399) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
