The APOB Polyclonal Antibody (CAB1330) is a valuable tool for researchers studying the apolipoprotein B (APOB) protein, which plays a crucial role in lipid metabolism and cardiovascular health. This antibody, produced in rabbits, is highly specific to human samples and is optimized for use in Western blot applications.APOB is a key component of lipoproteins, serving as the structural protein for chylomicrons, VLDL, IDL, and LDL particles. Dysregulation of APOB levels can lead to conditions such as hypercholesterolemia and atherosclerosis, making it an important target for therapeutic intervention.
By targeting APOB with this polyclonal antibody, researchers can accurately detect and quantify the protein in various cell types and tissues, providing valuable insights into lipid metabolism and cardiovascular disease. This antibody is a valuable tool for studies investigating the role of APOB in health and disease, advancing our understanding of lipid metabolism and potential therapeutic targets for cardiovascular conditions.
Product Name:
ApoB Rabbit Polyclonal Antibody
SKU:
CAB1330
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human ApoB (NP_000375.3).
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL), and is the ligand for the LDL receptor. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Purification Method:
Affinity purification
Gene ID:
338
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using ApoB Rabbit pAb (CAB1330) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
