The APLF Polyclonal Antibody (CAB17842) is specifically designed for research involving APLF, a protein implicated in DNA repair and maintenance of genomic stability. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. The APLF Polyclonal Antibody binds specifically to the APLF protein, allowing for the detection and analysis of this important molecule in various cell types.APLF (Aprataxin and PNK-like factor) is a key player in the DNA damage response pathway, specifically in the repair of DNA double-strand breaks. Dysfunction of APLF has been linked to various diseases, including cancer and neurological disorders, highlighting its importance in maintaining cellular integrity.
Research on APLF provides insight into the mechanisms behind DNA repair and could lead to the development of new therapeutic strategies for diseases associated with genomic instability.Overall, the APLF Polyclonal Antibody is a valuable tool for scientists conducting research in the fields of DNA repair, genomics, and disease pathology. Its high reactivity and specificity make it an ideal choice for studies aimed at understanding the role of APLF in maintaining genomic stability and preventing disease progression.
Product Name:
APLF Rabbit Polyclonal Antibody
SKU:
CAB17842
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human APLF (NP_775816.1).
Enables DNA-(apurinic or apyrimidinic site) endonuclease activity; nuclease activity; and nucleotide binding activity. Involved in double-strand break repair via nonhomologous end joining and single strand break repair. Acts upstream of or within positive regulation of DNA ligation. Located in nucleoplasm. Is active in site of double-strand break.
Purification Method:
Affinity purification
Gene ID:
200558
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung, using APLF Rabbit pAb (CAB17842) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
