The APITD1 Polyclonal Antibody (CAB8293) is a valuable tool for researchers studying the APITD1 protein, a key player in apoptosis, cell proliferation, and cancer development. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications. By binding to the APITD1 protein, this antibody enables the detection and analysis of APITD1 in various cell types, providing valuable insights for studies in cancer research and cell biology.
APITD1, also known as apoptosis-inducing, tumor suppressor P53-target, plays a crucial role in the regulation of cell death and survival pathways. Its involvement in apoptotic processes and tumor suppression makes it a promising target for research into cancer therapy and drug development. Understanding the function of APITD1 is essential for unraveling its role in cancer progression and identifying potential therapeutic interventions that target this protein.
Product Name:
APITD1 Rabbit Polyclonal Antibody
SKU:
CAB8293
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human APITD1 (NP_954988.1).
This gene was identified in the neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumors, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. The protein is a component of multiple complexes, including the Fanconi anemia (FA) core complex, the APITD1/CENPS complex, and the CENPA-CAD (nucleosome distal) complex. Known functions include an involvement with chromatin associations of the FA core complex, and a role in the stable assembly of the outer kinetochore. Alternative splicing of this gene results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream cortistatin (CORT) gene, as represented in GeneID:100526739. An APITD1-related pseudogene has been identified on chromosome 7.
Purification Method:
Affinity purification
Gene ID:
378708
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using APITD1 antibody (CAB8293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
