The APH1A Polyclonal Antibody (CAB14666) is a valuable tool for researchers studying APH1A, a key component of the gamma-secretase complex involved in cleavage of amyloid precursor protein and Notch receptors. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunohistochemistry applications.APH1A is essential for the function of the gamma-secretase complex, which plays a crucial role in various cellular processes including cell signaling, membrane protein processing, and neurogenesis. Dysregulation of gamma-secretase activity has been implicated in Alzheimer's disease, making APH1A a potential therapeutic target for this devastating neurodegenerative disorder.
The APH1A Polyclonal Antibody enables researchers to study the expression and localization of APH1A in different tissues and cell types, providing insights into its involvement in pathological pathways and potential for therapeutic intervention. By understanding the function of APH1A, researchers can advance our knowledge of neurodegenerative diseases and potentially develop new treatments to target gamma-secretase dysfunction.
Product Name:
APH1A Rabbit Polyclonal Antibody
SKU:
CAB14666
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human APH1A (NP_001071096.1).
This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants.
Purification Method:
Affinity purification
Gene ID:
51107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using APH1A Rabbit pAb (CAB14666) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
