The APEX1 Polyclonal Antibody (CAB1117) is a valuable tool for researchers studying APEX1, a multifunctional protein involved in DNA repair and redox signaling processes. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By binding to the APEX1 protein, this antibody enables the detection and analysis of APEX1 in various cellular contexts, making it an essential reagent for studies in DNA repair mechanisms, oxidative stress response, and cancer biology.APEX1, also known as APE1 or Ref-1, is a key player in maintaining genomic stability and regulating gene expression through its dual functions in DNA repair and transcriptional regulation.
Its role in repairing DNA damage and modulating redox signaling pathways highlights its importance in cellular homeostasis and disease development, particularly in cancer and neurodegenerative disorders. Investigating the functions of APEX1 is crucial for understanding its potential as a therapeutic target in diseases characterized by DNA damage and oxidative stress.
Product Name:
APEX1/APE1 Rabbit Polyclonal Antibody
SKU:
CAB1117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1/APE1 (NP_542380.1).
The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.
Purification Method:
Affinity purification
Gene ID:
328
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using APEX1/APE1 Rabbit pAb (CAB1117) .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
