The APEH Polyclonal Antibody (CAB5893) is a valuable tool for researchers studying APEH, a key enzyme involved in the hydrolysis of acyl-protein thioesters. The antibody is produced in rabbits and exhibits high reactivity towards human samples, making it suitable for use in Western blot applications. By targeting the APEH protein, this antibody allows for the precise detection and analysis of APEH in various cell types, making it an essential component for studies focused on enzymatic activity and its role in cellular processes.APEH, also known as acylpeptide hydrolase, plays a critical role in the regulation of protein degradation and turnover within the cell.
Dysregulation of APEH activity has been linked to various diseases, including neurodegenerative disorders and cancer. By understanding the mechanisms by which APEH functions, researchers can gain valuable insights into disease pathogenesis and potentially identify new therapeutic targets for intervention.Overall, the APEH Polyclonal Antibody (CAB5893) is a valuable tool for researchers looking to investigate the role of APEH in health and disease. Its high specificity and sensitivity make it an ideal choice for studies aimed at unraveling the complex functions of this important enzyme in cellular biology.
Product Name:
APEH Rabbit Polyclonal Antibody
SKU:
CAB5893
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
327
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] APEH Rabbit pAb (CAB5893) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
