The APC Rat IgG2a Isotype Control (B39-4) AGEL2894 is an essential tool for flow cytometry experiments requiring a matched isotype control for rat monoclonal antibodies. This control antibody, produced in rats, provides a baseline for comparison with specific rat IgG2a antibodies to accurately assess non-specific binding and background signal.Flow cytometry is a powerful technique used in immunology research to analyze and quantify cell populations based on surface markers. By using the APC Rat IgG2a Isotype Control (B39-4) AGEL2894 alongside experimental antibodies, researchers can differentiate true positive signals from noise and confidently interpret their flow cytometry results.
With its specificity for rat IgG2a, this isotype control ensures reliable and reproducible flow cytometry data by accounting for any non-specific interactions with cells or reagents. Its use is critical for distinguishing true antigen-specific staining from background staining, ultimately leading to accurate and meaningful conclusions in immunological studies.For researchers working with rat monoclonal antibodies in flow cytometry experiments, the APC Rat IgG2a Isotype Control (B39-4) AGEL2894 is a valuable resource that enhances the reliability and interpretability of their results, ultimately advancing our understanding of immune cell populations and their functions.
Product Name:
APC Rat IgG2a, λ Isotype Control [B39-4] (AGEL2894)
Product Code:
AGEL2894
Clone No:
B39-4
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2a, λ
Conjugation:
APC
Form:
Liquid
Fluorescent Label:
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.