The Mouse Anti-CD25 Antibody (PC61.5.3) from Assay Genie is a highly specific monoclonal antibody designed for research involving CD25, a key marker for regulatory T cells. This antibody, generated against the mouse CD25 protein, is ideal for flow cytometry and immunohistochemistry applications in mouse samples. CD25, also known as the interleukin-2 receptor alpha chain, plays a crucial role in immune regulation by promoting the activation and proliferation of T cells.
Research on CD25 is essential for understanding immune tolerance, autoimmune diseases, and the development of targeted immunotherapies. With its high specificity and validated performance, the Mouse Anti-CD25 Antibody (PC61.5.3) is a valuable tool for immunologists and researchers studying T cell biology and immunotherapy.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.