APAF1 Rabbit Polyclonal Antibody (CAB0751)
- SKU:
- CAB0751
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
Product Name: | APAF1 Rabbit Polyclonal Antibody |
SKU: | CAB0751 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-600 of human APAF1 (NP_863651.1). |
Sequence: | PHTL SPDQ EDCM YWYN FLAY HMAS AKMH KELC ALMF SLDW IKAK TELV GPAH LIHE FVEY RHIL DEKD CAVS ENFQ EFLS LNGH LLGR QPFP NIVQ LGLC EPET SEVY QQAK LQAK QEVD NGML YLEW INK |
Tested Applications: | WB IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
Synonyms: | CED4; APAF-1; APAF1 |
Positive Sample: | Hela,Jurkat,293T,Mouse testis |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm. |
Calculated MW: | 142kDa |
Observed MW: | 150kDa |
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Purification Method: | Affinity purification |
Gene ID: | 317 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |