APAF1 Antibody (PACO06254)
- SKU:
- PACO06254
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
APAF1 Antibody (PACO06254)
The APAF1 Antibody (PACO06254) is a valuable tool for research involving APAF1, a protein involved in regulating apoptosis or programmed cell death. This polyclonal antibody, generated in rabbits, is highly specific and reactive with human samples, making it suitable for various applications such as Western blotting and immunohistochemistry.APAF1, also known as apoptotic protease activating factor 1, plays a crucial role in the intrinsic apoptotic pathway by forming a complex with cytochrome c and caspase-9, ultimately leading to cell death.
Dysregulation of APAF1 expression has been linked to numerous diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.By using the APAF1 Antibody, researchers can study the role of APAF1 in apoptosis and its potential as a therapeutic target in diseases characterized by aberrant cell death. This antibody enables precise detection and analysis of APAF1 in various cell types, providing valuable insights into apoptotic signaling pathways and potential treatment strategies.
| Antibody Name: | APAF1 Antibody (PACO06254) |
| Antibody SKU: | PACO06254 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:10000, WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000 |
| Species Reactivity: | Human |
| Immunogen: | Synthesized peptide derived from the C-terminal region of human Apaf-1-ALT. |
| Form: | Liquid |
| Storage Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot analysis of Jurkat cells using Apaf-1-ALT Polyclonal Antibody. |
 | Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody. |
| Synonyms: | APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19; dJ179L10.2; likely ortholog of mouse monocyte macrophage 19 |
| UniProt Protein Function: | APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.Protein type: ApoptosisChromosomal Location of Human Ortholog: 12q23.1Cellular Component: apoptosome; cytosolMolecular Function: nucleotide binding; protein bindingBiological Process: apoptosis; caspase activation; caspase activation via cytochrome c; nervous system development; positive regulation of apoptosis; regulation of apoptosis |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14727-6 |
| NCBI GenInfo Identifier: | 20141188 |
| NCBI Gene ID: | 317 |
| NCBI Accession: | O14727.2 |
| UniProt Secondary Accession: | O14727-6,O43297, Q7Z438, Q9BXZ6, Q9UBZ5, Q9UGN8, Q9UGN9 Q9UGP0, Q9UJ58, Q9UJ59 |
| UniProt Related Accession: | O14727-6,O14727 |
| Molecular Weight: | 37,976 Da |
| NCBI Full Name: | Apoptotic protease-activating factor 1 |
| NCBI Synonym Full Names: | apoptotic peptidase activating factor 1 |
| NCBI Official Symbol: | APAF1Â Â |
| NCBI Official Synonym Symbols: | CED4; APAF-1Â Â |
| NCBI Protein Information: | apoptotic protease-activating factor 1 |
| UniProt Protein Name: | Apoptotic protease-activating factor 1 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Apoptotic protease-activating factor |
| UniProt Gene Name: | APAF1Â Â |
| UniProt Entry Name: |
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