Anti-AP4B1 Antibody (PACO42882)

AP4B1 Antibody (PACO42882)

SKU:

PACO42882

Product Type:

Antibody

Reactivity:

Human

Host Species:

Rabbit

Isotype:

IgG

Applications:

ELISA

IHC

Antibody Type:

Polyclonal Antibody

Conjugation:

Unconjugated

Description

Description

DatasheetMSDS

AP4B1 Antibody (PACO42882)

The AP4B1 Polyclonal Antibody (PAC042882) is a valuable tool for researchers studying AP4B1, a crucial component of the adaptor protein complex 4 (AP-4) involved in intracellular vesicle trafficking. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the AP4B1 protein, researchers can easily detect and analyze its expression in a variety of cell types, making it ideal for studies in cell biology and molecular biology.

AP4B1 plays a vital role in the sorting and trafficking of cellular proteins, particularly in the biogenesis of lysosome-related organelles. Dysregulation of AP4B1 has been linked to various genetic disorders and neurodevelopmental conditions, making it an attractive target for research in these areas. Understanding the function of AP4B1 is essential for unraveling its role in cellular pathways and developing potential therapeutic interventions for related diseases.

RIMS4 Antibody (PACO64925)

$383

Product Code:

PACO64925

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Western Blot<br />
Positive WB detected in: Mouse heart tissue,Mouse kidney tissue<br />
All lanes: ompF antibody at 1:2000<br />
Secondary<br />
Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
Predicted band size: 57 kDa<br />
Observed band size: 57 kDa<br /> — Choose Options

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Ang4 Antibody (PACO64593)

$407

Product Code:

PACO64593

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Immunofluorescence staining of NIH/3T3 cells with PACO64869 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). — Choose Options

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OR13C8 Antibody (PACO64869)

$383

Product Code:

PACO64869

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Immunofluorescence staining of Hela cells with PACO64949 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). — Choose Options

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SIM1 Antibody (PACO64949)

$407

Product Code:

PACO64949

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Western Blot<br />
Positive WB detected in: Escherichia coli lysate<br />
All lanes: OTSB antibody at 1:2000<br />
Secondary<br />
Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
Predicted band size: 30 kDa<br />
Observed band size: 30 kDa<br /> — Choose Options

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otsB Antibody (PACO64885)

$407

Product Code:

PACO64885

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

IHC image of PACO64929 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. — Choose Options

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RNF122 Antibody (PACO64929)

$383

Product Code:

PACO64929

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Immunofluorescence staining of SH-SY5Y cells with PACO64589-IF at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). — Choose Options

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AGO4 Antibody (PACO64589)

$383

Product Code:

PACO64589

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

IHC image of PACO64729 diluted at 1:200 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. — Choose Options

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Fn1 Antibody (PACO64729)

$407

Product Code:

PACO64729

Isotype:

IgG

IHC image of PACO64713 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. — Choose Options

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FAM53A Antibody (PACO64713)

$407

Product Code:

PACO64713

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Antibody Name:	AP4B1 Antibody (PACO42882)
Antibody SKU:	PACO42882
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human AP-4 complex subunit beta-1 protein (201-500AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Background:	Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
Synonyms:	AP-4 complex subunit beta-1 (AP-4 adaptor complex subunit beta) (Adaptor-related protein complex 4 subunit beta-1) (β subunit of AP-4) (Beta4-adaptin), AP4B1

UniProt Protein Function:	AP4B1: Subunit of novel type of clathrin- or non-clathrin- associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. Defects in AP4B1 are the cause of cerebral palsy spastic quadriplegic type 5 (CPSQ5). CPSQ5 is a neurodevelopmental disorder characterized by neonatal hypotonia that progresses to hypertonia and spasticity, and severe mental retardation with poor or absent speech development. Belongs to the adaptor complexes large subunit family.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 1p13.2Cellular Component: trans-Golgi network; trans-Golgi network membraneMolecular Function: protein binding; transporter activityDisease: Spastic Paraplegia 47, Autosomal Recessive
UniProt Protein Details:
NCBI Summary:	This gene encodes a subunit of a heterotetrameric adapter-like complex 4 that is involved in targeting proteins from the trans-Golgi network to the endosomal-lysosomal system. Mutations in this gene are associated with cerebral palsy spastic quadriplegic type 5 (CPSQ5) disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9Y6B7
NCBI GenInfo Identifier:	126302520
NCBI Gene ID:	10717
NCBI Accession:	Q9Y6B7.2
UniProt Secondary Accession:	Q9Y6B7,Q59EJ4, Q96CL6, B7Z4X3
UniProt Related Accession:	Q9Y6B7
Molecular Weight:	34,403 Da
NCBI Full Name:	AP-4 complex subunit beta-1
NCBI Synonym Full Names:	adaptor related protein complex 4 beta 1 subunit
NCBI Official Symbol:	AP4B1
NCBI Official Synonym Symbols:	CPSQ5; SPG47; BETA-4
NCBI Protein Information:	AP-4 complex subunit beta-1
UniProt Protein Name:	AP-4 complex subunit beta-1
UniProt Synonym Protein Names:	AP-4 adaptor complex subunit beta; Adaptor-related protein complex 4 subunit beta-1; Beta subunit of AP-4; Beta4-adaptin
Protein Family:	AP-4 complex
UniProt Gene Name:	AP4B1
UniProt Entry Name:	AP4B1_HUMAN

AP4B1 Antibody (PACO42882)

Description

AP4B1 Antibody (PACO42882)

Overview

Properties

Product Images

Additional Information

Related Products

	Western blot. All lanes: AP4B1 antibody at 3µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: U251 whole cell lysate. Lane 3: LO2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 84, 35 kDa. Observed band size: 84 kDa.
	Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO42882 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human testis tissue using PACO42882 at dilution of 1:100.

AP4B1 Antibody (PACO42882)

Description

AP4B1 Antibody (PACO42882)

Overview

Properties

Product Images

Additional Information

Related Products

Related Products

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