The AP2M1 Polyclonal Antibody (CAB13962) is a valuable tool for researchers studying the role of AP2M1, a key protein involved in clathrin-mediated endocytosis. This polyclonal antibody, raised in rabbits, is highly specific for detecting AP2M1 in human samples and has been validated for use in Western blot applications. By binding to the AP2M1 protein, this antibody enables researchers to investigate the function and regulation of AP2M1 in various cell types, making it ideal for research in cell biology and membrane trafficking.AP2M1 is a subunit of the AP2 complex, which plays a crucial role in the internalization of cell surface receptors and the trafficking of proteins within cells.
Dysregulation of AP2M1 has been associated with various diseases, including cancer and neurodegenerative disorders. By studying the function of AP2M1, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the AP2M1 Polyclonal Antibody is a valuable tool for researchers investigating the role of AP2M1 in cellular processes and disease pathogenesis. Its high specificity and sensitivity make it a reliable choice for studying the function of AP2M1 in a variety of research applications.
Product Name:
AP2M1 Rabbit Polyclonal Antibody
SKU:
CAB13962
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human AP2M1 (NP_004059.2).
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1173
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AP2M1 antibody (CAB13962) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
