The AP2B1 Polyclonal Antibody (CAB1995) is a valuable tool for researchers studying the AP2B1 protein, a key component of the adaptor protein complex 2 (AP-2) involved in clathrin-mediated endocytosis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.AP2B1 is essential for the internalization of cell surface receptors and the transport of cargo proteins in vesicles, making it a critical player in cellular trafficking processes. By targeting AP2B1 with this antibody, researchers can detect and analyze the protein in various cell types, aiding in the investigation of endocytic pathways and their role in cellular function.
With its ability to specifically bind to AP2B1, this antibody is ideal for studies in cell biology, membrane trafficking, and signal transduction. Understanding the function of AP2B1 is fundamental for unraveling the complexities of intracellular transport mechanisms and their implications in health and disease. Researchers can utilize this antibody to deepen their understanding of AP2B1's role in processes such as receptor recycling, protein sorting, and membrane dynamics.
Product Name:
AP2B1 Rabbit Polyclonal Antibody
SKU:
CAB1995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
163
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using AP2B1 Rabbit pAb (CAB1995) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
