The AP2A2 Polyclonal Antibody (CAB5391) is a valuable tool for researchers studying the AP2A2 protein, which is involved in the clathrin-mediated endocytosis pathway. This antibody is produced in rabbits and is highly specific for human samples, making it an ideal choice for Western blot experiments.AP2A2 is a key player in the process of internalizing cell surface receptors and other molecules, regulating important cellular functions such as signaling and nutrient uptake. By targeting the AP2A2 protein, researchers can gain insights into the mechanisms of endocytosis and its role in various physiological processes.
With its high reactivity and specificity, the AP2A2 Polyclonal Antibody is a valuable tool for studies in cell biology, molecular biology, and pharmacology. Researchers can use this antibody to detect and analyze AP2A2 expression in different cell types and tissues, providing valuable information for understanding the intricate processes of endocytosis and its implications in health and disease.
Product Name:
AP2A2 Rabbit Polyclonal Antibody
SKU:
CAB5391
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human Alpha2-adaptin/AP2A2 (NP_036437.1).
The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
Purification Method:
Affinity purification
Gene ID:
161
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Alpha2-adaptin/AP2A2 antibody (CAB5391) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
