The AP2A1 Polyclonal Antibody (CAB6863) is a valuable tool for researchers studying AP2A1, a protein involved in clathrin-mediated endocytosis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AP2A1 protein, this antibody enables the detection and analysis of AP2A1 in a variety of cell types, making it ideal for studies in cell biology and molecular biology.
AP2A1 is a key player in the process of clathrin-mediated endocytosis, which is essential for the internalization of cell surface receptors and various cargo molecules. Dysregulation of this process has been implicated in diseases such as cancer, infectious diseases, and neurodegenerative disorders. Research into the function and regulation of AP2A1 is crucial for understanding these diseases and developing targeted therapies to modulate endocytosis for therapeutic purposes.
Product Name:
AP2A1 Rabbit Polyclonal Antibody
SKU:
CAB6863
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AP2A1 (NP_055018.2).
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.
Purification Method:
Affinity purification
Gene ID:
160
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AP2A1 antibody (CAB6863) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
