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AP2A1 Rabbit Monoclonal Antibody (CAB4403)

SKU:
CAB4403
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:AP2A1 Rabbit Monoclonal Antibody
SKU:CAB4403
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human AP2A1 (O95782).
Sequence:MPAV SKGD GMRG LAVF ISDI RNCK SKEA EIKR INKE LANI RSKF KGDK ALDG YSKK KYVC KLLF IFLL GHDI DFGH MEAV NLLS SNKY TEKQ IGYL FISV
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:ADTAA; CLAPA1; AP2-ALPHA; AP2A1
Positive Sample:MCF7,U-251MG,Mouse testis,Mouse brain,Rat brain
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit.
Calculated MW:108kDa
Observed MW:100-108kDa

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Purification Method:Affinity purification
Gene ID:160
Clone Number:ARC0998
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using AP2A1 Rabbit mAb (CAB4403) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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