Zyxin Rabbit Monoclonal Antibody (CAB2298)
- SKU:
- CAB2298
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- ESP-2
- HED-2
- Research Area:
- Cell Biology
Description
| Product Name: | Zyxin Rabbit Monoclonal Antibody |
| SKU: | CAB2298 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Zyxin (Q15942). |
| Sequence: | TQPR GPPA SSPA PAPK FSPV TPKF TPVA SKFS PGAP GGSG SQPN QKLG HPEA LSAG TGSP QPPS FTYA QQRE KPRV QEKQ HPVP PPAQ NQNQ VRSP GAPG P |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 |
| Synonyms: | ESP-2; HED-2; Zyxin |
| Positive Sample: | MCF7,Mouse lung |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell junction, Cytoplasm, Nucleus, cytoskeleton, focal adhesion. |
| Calculated MW: | 61kDa |
| Observed MW: | 78kDa |
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
| Purification Method: | Affinity purification |
| Gene ID: | 7791 |
| Clone Number: | ARC1906 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Zyxin Rabbit mAb (CAB2298) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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