The YUCCA1 Polyclonal Antibody (CAB20733) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with plant samples and has been validated for use in various applications, including Western blotting.Yucca1 is involved in the biosynthesis of the plant hormone auxin, which regulates numerous processes such as cell elongation, root development, and fruit ripening. Understanding the function and regulation of Yucca1 is essential for unraveling the complex mechanisms underlying plant growth and response to environmental cues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
YUCCA1 Polyclonal Antibody
SKU:
CAB20733
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L23H3.20, L23H3_20, YUC, YUCCA, YUCCA 1, YUCCA1
Positive Sample:
Inflorescences, Before inflorescence, Rosette leaf
Calculated MW:
46kDa
Observed MW:
46kDa
Mutant has elevated levels of free IAA in dominant mutant allele; Flavin Monooxygenase-Like Enzyme; Auxin Biosynthesis
Purification Method
Affinity purification
Gene ID
829389
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using YUCCA1 Rabbit pAb (CAB20733) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
