The XIAP Polyclonal Antibody (CAB20846) is a valuable tool for researchers studying XIAP, a potent inhibitor of apoptosis protein that plays a crucial role in regulating cell death and survival pathways. This antibody, produced in knockout-validated rabbits, exhibits high specificity for XIAP in human samples and is validated for use in Western blot applications.XIAP, also known as X-linked inhibitor of apoptosis protein, is a key player in the apoptotic process, blocking the activation of caspases and preventing cell death. Dysregulation of XIAP has been implicated in various diseases, including cancer, making it an attractive target for therapeutic intervention.
By targeting XIAP, researchers can potentially develop novel treatments for cancer and other diseases where apoptosis evasion is a hallmark feature.The XIAP Polyclonal Antibody is a reliable tool for detecting and studying XIAP expression in various cell types, providing valuable insights into its function and dysregulation in disease. Its knockout validation ensures specificity and accuracy in experimental results, making it an essential reagent for studies in cancer biology and cell death mechanisms.
Product Name:
[KO Validated] XIAP Monoclonal Antibody
SKU:
CAB20846
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2).
API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3; AP
Positive Sample:
HeLa,RAW 264.7,PC-12
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
53kDa
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
Purification Method:
Affinity purification
Gene ID:
331
Clone Number:
ARC51953
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using XIAP antibody (CAB20846) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
