The Anti-WRAP53 Antibody (CAB20167) is a valuable tool for researchers studying the WRAP53 protein, which plays a crucial role in DNA repair and telomere maintenance. This polyclonal antibody is generated in rabbits and exhibits high reactivity with human samples, making it ideal for use in immunofluorescence and immunoprecipitation techniques. By binding to WRAP53, the antibody allows for the detection and analysis of this protein in a variety of cell types, supporting studies in DNA repair, telomere biology, and cancer research.WRAP53, also known as WD40-encoding RNA antisense to p53, functions as a scaffold protein that helps recruit factors involved in DNA repair pathways to sites of DNA damage.
It is also essential for telomerase function and telomere length maintenance, making it a key player in cell survival and genome stability. Research into WRAP53 is important for understanding its role in diseases such as cancer, where abnormalities in DNA repair and telomere maintenance can lead to genomic instability and tumorigenesis.Overall, the Anti-WRAP53 Antibody is a valuable tool for researchers investigating the functions of WRAP53 in DNA repair, telomere biology, and cancer development. Its high specificity and sensitivity make it a reliable reagent for experimental studies aimed at unraveling the mechanisms by which WRAP53 influences cellular processes and disease pathways.
Product Name:
WRAP53 Rabbit Polyclonal Antibody
SKU:
CAB20167
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 62-160 of human WRAP53 (NP_060551.2).
This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
55135
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse kidney, using WRAP53 Rabbit pAb (CAB20167) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
