The VPS24 Polyclonal Antibody (CAB0941) is a valuable tool for researchers studying VPS24, a protein involved in the endosomal sorting complex required for transport (ESCRT) pathway. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the VPS24 protein, this antibody allows for the detection and analysis of VPS24 in various cell types, making it essential for studies in cell biology, endosomal trafficking, and diseases related to ESCRT dysfunction.
VPS24, a key component of the ESCRT-III complex, plays a crucial role in the sorting and degradation of cellular proteins, membrane repair, and viral budding. Dysregulation of the ESCRT pathway, including VPS24, has been linked to various diseases such as neurodegeneration, cancer, and viral infections. By understanding the function of VPS24, researchers can gain insights into disease mechanisms and develop targeted therapies to treat ESCRT-related disorders.
Product Name:
VPS24 Rabbit Monoclonal Antibody
SKU:
CAB0941
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 65-220 of human VPS24 (Q9Y3E7).
Cytoplasm, Endosome, Late endosome membrane, Lipid-anchor, Membrane, cytosol.
Calculated MW:
25kDa
Observed MW:
29kDa
This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene.
Purification Method:
Affinity purification
Gene ID:
51652
Clone Number:
ARC1849
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using VPS24 Rabbit mAb (CAB0941) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
