The Anti-VPS18 Antibody (CAB17563) is a valuable tool for researchers studying the function of VPS18, a key component of the class C core vacuole/endosome tethering (CORVET) complex involved in membrane trafficking and fusion processes. This polyclonal antibody, generated in rabbits, is highly specific for VPS18 and is suitable for use in various experimental techniques, including Western blotting and immunofluorescence.VPS18 plays a crucial role in endosomal sorting and autophagy, making it an essential player in cellular homeostasis and protein degradation pathways.
Dysregulation of VPS18 function has been implicated in various diseases, including neurodegenerative disorders and cancer. By using the Anti-VPS18 Antibody, researchers can investigate the localization and expression of VPS18 in different cell types, thereby advancing our understanding of its physiological functions and potential therapeutic applications.
Product Name:
VPS18 Rabbit Monoclonal Antibody
SKU:
CAB17563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253).
Autophagosome, Clathrin-coated vesicle, CORVET complex, Early endosome, endosome, Glutamatergic synapse, Late endosome, Lysosome, Presynapse
Calculated MW:
110kDa
Observed MW:
110kDa
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Purification Method:
Affinity purification
Gene ID:
57617
Clone Number:
ARC2156
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using VPS18 Rabbit mAb (CAB17563) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
