The Anti-UCP1/UCP3 Antibody (CAB19526) is a valuable tool for researchers studying uncoupling proteins 1 and 3, which are involved in thermogenesis and energy metabolism in mitochondria. This polyclonal antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. It binds to both UCP1 and UCP3 proteins, allowing for accurate detection and analysis in various cell types.
UCP1 and UCP3 are key regulators of energy expenditure and have been implicated in metabolic disorders such as obesity and diabetes. By targeting these proteins, researchers can gain insights into how they function and potentially identify novel therapeutic strategies for these conditions. The Anti-UCP1/UCP3 Antibody is an essential tool for investigating the role of uncoupling proteins in metabolic health and disease.
Product Name:
UCP1+UCP3 Rabbit Monoclonal Antibody
SKU:
CAB19526
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of mouse UCP1+UCP3 (P12242).
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
Purification Method:
Affinity purification
Gene ID:
22227/22229
Clone Number:
ARC2304
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using UCP1+UCP3 Rabbit mAb (CAB19526) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
