UCP1 Polyclonal Antibody (CAB18853)
- SKU:
- CAB18853
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Porcine
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Product Name: | UCP1 Polyclonal Antibody |
| SKU: | CAB18853 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of cattle UCP1 (NP_001160000.1). |
| Sequence: | YDTV QEFF TTGK EASL GSKI SAGL MTGG VAVF IGQP TEVV KVRL QAQS HLHG PKPR YTGT YNAY RIIA TTEG LTGL WKGT TPNL TRNV IINC TELV TYDL M |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | Mitochondrial brown fat uncoupling protein 1 |
| Positive Sample: | Mouse heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Mitochondrion inner membrane |
| Calculated MW: | 33kDa |
| Observed MW: | 30kDa |
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
| Purification Method: | Affinity purification |
| Gene ID: | 281561 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Mouse heart, using UCP1 Rabbit pAb (CAB18853) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s. |
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