The UCP1 Polyclonal Antibody (CAB18853) is a valuable tool for research focused on UCP1, a protein found in brown adipose tissue that plays a key role in thermogenesis and energy expenditure. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting UCP1 in various experimental settings, including immunohistochemistry and Western blot analysis.UCP1, also known as uncoupling protein 1, is a mitochondrial protein that facilitates the uncoupling of oxidative phosphorylation from ATP production, leading to the generation of heat instead of energy storage.
Its importance in regulating metabolic rate and body temperature makes it a promising target for investigating obesity, diabetes, and other metabolic disorders.By using the UCP1 Polyclonal Antibody, researchers can gain valuable insights into the expression and function of UCP1 in different tissues and experimental models. This antibody is a valuable tool for studies aiming to understand the molecular mechanisms underlying thermogenesis and energy metabolism, with potential implications for the development of novel therapeutic strategies targeting metabolic diseases and obesity.
Product Name:
UCP1 Polyclonal Antibody
SKU:
CAB18853
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of cattle UCP1 (NP_001160000.1).
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
Purification Method:
Affinity purification
Gene ID:
281561
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using UCP1 Rabbit pAb (CAB18853) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
