The Anti-Ube4A Antibody (CAB0710) offered by Assay Genie is a highly specific tool for research involving the Ube4A protein, a key player in protein degradation and regulation processes within cells. This polyclonal antibody, derived from rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the Ube4A protein, this antibody enables the detection and analysis of Ube4A in various cell types, making it an essential tool for studies in molecular biology and cellular pathway regulation.
Ube4A, also known as a ubiquitin ligase enzyme, plays a crucial role in tagging proteins for degradation and regulating cellular processes such as protein turnover and quality control. Dysregulation of Ube4A has been implicated in various diseases, including neurodegenerative disorders and cancer. By understanding the function and activity of Ube4A, researchers can potentially unravel new insights into disease mechanisms and identify novel therapeutic targets aimed at restoring proper cellular function.
Product Name:
UBE4A Rabbit Monoclonal Antibody
SKU:
CAB0710
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4A (Q14139).
PUMC-HUVEC-T1,Mouse brain, Mouse kidney,Rat liver, Rat brain
Conjugate:
Unmodified
Cellular Localization:
Cytoplasm.
Calculated MW:
123kDa
Observed MW:
125kDa
This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
9354
Clone Number:
ARC2534
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HUVEC cells, using UBE4A antibody (CAB0710) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
