The UAP1 Polyclonal Antibody (CAB8662) is a crucial tool for researchers studying UAP1, a key enzyme involved in the biosynthesis of UDP-N-acetylglucosamine, which is essential for protein glycosylation. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the UAP1 protein, it allows for accurate detection and analysis in a variety of cell types, enabling in-depth investigations into glycosylation pathways and related cellular processes.
UAP1, also known as UDP-N-acetylglucosamine pyrophosphorylase 1, is a critical enzyme in the synthesis of UDP-GlcNAc, a sugar nucleotide required for the modification of proteins with N-linked glycosylation. Dysregulation of this process has been linked to various human diseases, including cancer, diabetes, and congenital disorders affecting glycosylation. The UAP1 antibody provides valuable insights into the function and regulation of this enzyme, facilitating research into potential therapeutic strategies targeting glycosylation pathways.
Product Name:
UAP1 Rabbit Monoclonal Antibody
SKU:
CAB8662
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human UAP1 (Q16222).
Enables identical protein binding activity. Predicted to be involved in UDP-N-acetylglucosamine biosynthetic process. Located in cytosol; nucleoplasm; and plasma membrane.
Purification Method:
Affinity purification
Gene ID:
6675
Clone Number:
ARC1769
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using UAP1 Rabbit mAb (CAB8662) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
