The Anti-TWIST Antibody (CAB15596) is a highly specific tool for research involving TWIST, a transcription factor known to play a crucial role in cell differentiation and development. This polyclonal antibody, developed and validated using knockout (KO) models, is ideal for investigating the functions and regulatory mechanisms of TWIST in various biological processes.TWIST is a key regulator of epithelial-mesenchymal transition (EMT), a process involved in embryonic development, tissue regeneration, and cancer metastasis. Dysregulation of TWIST expression has been linked to various diseases, including cancer and genetic disorders.
By targeting TWIST with this antibody, researchers can gain valuable insights into its role in these conditions and potentially identify novel therapeutic strategies.The Anti-TWIST Antibody is suitable for use in immunofluorescence, immunohistochemistry, and other experimental techniques, allowing for detailed localization and quantification of TWIST expression in cells and tissues. Its high specificity and sensitivity make it a valuable tool for researchers studying the molecular mechanisms underlying TWIST-mediated processes.
Product Name:
[KO Validated] Twist Rabbit Polyclonal Antibody
SKU:
CAB15596
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 51-150 of human Twist (NP_000465.1).
This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.
Purification Method:
Affinity purification
Gene ID:
7291
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Twist Rabbit pAb (CAB15596) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
