The TRUB1 Polyclonal Antibody (CAB20492) is a valuable tool for researchers studying TRUB1, an enzyme involved in RNA modification processes. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to the TRUB1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent choice for investigations in molecular biology and RNA biology.TRUB1, also known as tRNA pseudouridine synthase B, plays a crucial role in catalyzing the formation of pseudouridine in tRNA molecules, which is essential for proper tRNA function.
Dysregulation of TRUB1 activity has been implicated in various diseases, including cancer and neurological disorders. By studying the function of TRUB1, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention. Understanding the role of TRUB1 in RNA modification pathways is vital for advancing our knowledge of gene expression and its implications for human health.
Product Name:
TRUB1 Rabbit Polyclonal Antibody
SKU:
CAB20492
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TRUB1 (NP_631908.1).
Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).
Purification Method:
Affinity purification
Gene ID:
142940
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRUB1 Rabbit pAb (CAB20492) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
