The Anti-Troponin I1 (TNI1) Antibody (CAB9664) is a highly specific tool for researchers studying troponin I, a key protein involved in muscle contraction and a sensitive biomarker of cardiac injury. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.Troponin I1 is a cardiac-specific isoform that is released into the bloodstream following myocardial damage, making it a critical marker for diagnosing heart attacks and other cardiac conditions.
By targeting troponin I1, researchers can gain insights into cardiac function, injury mechanisms, and potential therapeutic interventions related to heart disease.With its high specificity and reliability, the Anti-Troponin I1 (TNI1) Antibody (CAB9664) is an essential tool for investigations into cardiac biomarkers, cardiovascular diseases, and other conditions affecting cardiac muscle. Its use can help further our understanding of heart health and contribute to the development of improved diagnostic tools and treatment strategies in the field of cardiology.
Product Name:
TNNI1 Rabbit Monoclonal Antibody
SKU:
CAB9664
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human Troponin I1/TnI1 (P19237).
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
Purification Method:
Affinity purification
Gene ID:
7135
Clone Number:
ARC1687
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat skeletal muscle, using Troponin I1/TnI1 antibody (CAB9664) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
