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TPO-R/CD110/c-Mpl Rabbit Monoclonal Antibody (CAB19729)

SKU:
CAB19729
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
C-MPL
CD110
MPLV
THCYT2
TPOR
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

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TPO-R/CD110/c-Mpl Rabbit Monoclonal Antibody (CAB19729)

The Anti-TPO (R) CD110 (c-Mpl) Antibody (CAB19729) is a valuable tool for researchers studying the CD110 (c-Mpl) protein, which plays a crucial role in immune regulation and hematopoiesis. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various research applications, including Western blot analysis.CD110 (c-Mpl) is a receptor for thrombopoietin, a cytokine involved in the production of platelets and regulation of hematopoietic stem cells. Dysregulation of the CD110 (c-Mpl) pathway has been implicated in various hematologic disorders, making it a key target for investigation in the fields of hematology and oncology.

By binding specifically to the CD110 (c-Mpl) protein, this antibody enables researchers to detect and analyze its expression in different cell types, providing valuable insights into its role in normal and pathological conditions. Understanding the function of CD110 (c-Mpl) is essential for developing targeted therapies for blood-related disorders and cancers.

Product Name:TPO-R/CD110/c-Mpl Rabbit Monoclonal Antibody
SKU:CAB19729
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 536-635 of human TPO-R/CD110/c-Mpl (P40238).
Sequence:HRVL GQYL RDTA ALSP PKAT VSDT CEEV EPSL LEIL PKSS ERTP LPLC SSQA QMDY RRLQ PSCL GTMP LSVC PPMA ESGS CCTT HIAN HSYL PLSY WQQP
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:MPLV; TPOR; C-MPL; CD110; THPOR; THCYT2; TPO-R/CD110/c-Mpl
Positive Sample:A-549,Raji,THP-1,Mouse liver,Mouse spleen,Mouse heart,Rat liver
Conjugate:Unconjugated
Cellular Localization:cell surface, external side of plasma membrane, Golgi apparatus, nuclear membrane, plasma membrane
Calculated MW:71kDa
Observed MW:80kDa

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Purification Method:Affinity purification
Gene ID:4352
Clone Number:ARC2257
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using TPO-R/CD110/c-Mpl Rabbit mAb (CAB19729) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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