The TPMT Polyclonal Antibody (CAB20922) is a valuable tool for researchers studying thiopurine S-methyltransferase (TPMT), an enzyme involved in the metabolism of thiopurine drugs used in the treatment of leukemia and autoimmune diseases. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blotting applications.TPMT plays a crucial role in determining the efficacy and toxicity of thiopurine medications, making it a focal point of study in pharmacogenetics and personalized medicine. Understanding the expression and activity of TPMT in different cell types and tissues is essential for optimizing drug dosages and minimizing adverse reactions in patients undergoing thiopurine therapy.
By detecting and quantifying TPMT protein levels, researchers can gain insights into drug metabolism, drug interactions, and individual variations in drug response. The TPMT Polyclonal Antibody is a reliable tool for investigating TPMT expression patterns in both normal and diseased tissues, with applications in drug development, clinical diagnostics, and precision medicine research.
Product Name:
TPMT Monoclonal Antibody
SKU:
CAB20922
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TPMT (P51580).
HeLa, 293T, Hep G2, K-562, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
28kDa
Observed MW:
28-32kDa
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.
Purification Method:
Affinity purification
Gene ID:
7172
Clone Number:
ARC2897
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TPMT antibody (CAB20922) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
