The Anti-TNF Antibody (CAB20851) is a valuable tool for researchers studying tumor necrosis factor (TNF), a proinflammatory cytokine involved in various immune responses and inflammatory conditions. This antibody, derived from rabbits, has high specificity and sensitivity for human samples, making it ideal for use in immunohistochemistry, ELISA, and Western blotting experiments.TNF is a key player in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis.
By targeting TNF, researchers can gain important insights into the mechanisms underlying these conditions and develop potential therapeutic strategies to modulate TNF activity. The Anti-TNF Antibody (CAB20851) provides a reliable tool for investigating TNF expression and function in various cell types and tissues, advancing our understanding of TNF-mediated immune responses and inflammatory processes.
Product Name:
TNF Polyclonal Antibody
SKU:
CAB20851
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-235 of mouse TNF-α (NP_038721.1).
cell surface, cytoplasm, external side of plasma membrane, extracellular region, extracellular space, phagocytic cup, plasma membrane, recycling endosome
Calculated MW:
26kDa
Observed MW:
25kDa/28kDa
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
21926
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW 264.7 cells using TNF-α Rabbit pAb (CAB20851) at 1:1000 dilution. Raw 264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
